Washing
of Wheat Grains
Boiling
of Wheat Grains till they Swell up
Remove
Excess water
Surface
Drying of Wheat Grains Chemical Mixing CaSO4 & CaCo3 (2% and 0.5%)
Bags
preparations (Polypropylene Bags)
Sterlized
at :
Temp-
127*c
Pressure-
22 psi
Time-
90 min.
Incubation
of Bags
Temp-
24-25*c
Time-
10-12 days
Media
Preparation
Mix
and heat till contents mix properly
Pouring
in test tubes
Sterilized
at
Temp-
121*c
Pressure-
15 psi
Time-
20 min.
Slant
Preparation
Inoculation (Transfer master spawn to spawn)
Spawn
Bag (1 kg)
