HOW TO MAKE MUSHROOM SPAWN IN A EASY WAY-INDIA

Washing of Wheat Grains

Boiling of Wheat Grains till they Swell up

Remove Excess water

Surface Drying of Wheat Grains Chemical Mixing CaSO4 & CaCo3 (2% and 0.5%)

Bags preparations (Polypropylene Bags)

Sterlized at :

Temp- 127*c

Pressure- 22 psi

Time- 90 min.

Incubation of Bags

Temp- 24-25*c

Time- 10-12 days

Media Preparation

Mix and heat till contents mix properly

Pouring in test tubes

Sterilized at

Temp- 121*c

Pressure- 15 psi

Time- 20 min.

Slant Preparation

Inoculation  (Transfer master spawn to spawn)

Spawn Bag (1 kg)