Wednesday, 13 March 2013

HOW TO MAKE MUSHROOM SPAWN IN A EASY WAY-INDIA








Washing of Wheat Grains


Boiling of Wheat Grains till they Swell up
Remove Excess water


Surface Drying of Wheat Grains Chemical Mixing CaSO4 & CaCo3 (2% and 0.5%)


Bags preparations (Polypropylene Bags)
Sterlized at :
Temp- 127*c
Pressure- 22 psi
Time- 90 min.



Incubation of Bags
Temp- 24-25*c
Time- 10-12 days


Media Preparation
Mix and heat till contents mix properly


Pouring in test tubes


Sterilized at
Temp- 121*c
Pressure- 15 psi
Time- 20 min.


Slant Preparation


Inoculation  (Transfer master spawn to spawn)


Spawn Bag (1 kg)


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